Spectroscopic characterization of the key catalytic intermediate Ni-C in the O2-tolerant [NiFe] hydrogenase I from Aquifex aeolicus: evidence of a weakly bound hydride

Spectroscopic characterization of the key catalytic intermediate Ni-C in the O2-tolerant [NiFe] hydrogenase I from Aquifex aeolicus: evidence of a weakly bound hydride:

Chem. Commun., 2012, DOI: 10.1039/C1CC16109A

Maria-Eirini Pandelia, Pascale Infossi, Matthias Stein, Marie-Therese Giudici-Orticoni, Wolfgang Lubitz
Ni-C in the O₂-tolerant [NiFe] hydrogenase from A. aeolicus is shown by pulse EPR to carry a photo-labile hydride ligand, which is weaker bound compared to O₂-sensitive hydrogenases.